Melbourne/Lab Notebook

From 2007.igem.org

(Difference between revisions)
(25 June 2007)
Line 2: Line 2:
==Week 1==
==Week 1==
===25 June 2007===
===25 June 2007===
 +
 +
Prepared LB agar plates.
 +
 +
====Transformation====
 +
#resuspended the following from Registry plates:
 +
**BBa_I15008 - ho1 (P2 21A)
 +
**BBa_I15009 - PcyA (P2 21C)
 +
**BBa_R0084 - OmpR positive promoter (P1 11H)
 +
##Punctured foil with pipette tip.
 +
##Resuspended in 15uL ddH2O.
 +
##Stored in-20 (after taking 1uL for transformation).
 +
#[[Melb:Transformation Protocol|Transformed]] into competent DH5alpha cells.
 +
===26 June 2007===
===26 June 2007===
===27 June 2007===
===27 June 2007===

Revision as of 00:25, 3 July 2007

<Back to team home page>

Contents

Week 1

25 June 2007

Prepared LB agar plates.

Transformation

  1. resuspended the following from Registry plates:
    • BBa_I15008 - ho1 (P2 21A)
    • BBa_I15009 - PcyA (P2 21C)
    • BBa_R0084 - OmpR positive promoter (P1 11H)
    1. Punctured foil with pipette tip.
    2. Resuspended in 15uL ddH2O.
    3. Stored in-20 (after taking 1uL for transformation).
  1. Transformed into competent DH5alpha cells.

26 June 2007

27 June 2007

28 June 2007

29 June 2007

30 June 2007

Week 2

2 July 2007

Now

3 July 2007

4 July 2007

5 July 2007

6 July 2007

Week 3

9 July 2007

10 July 2007

11 July 2007

12 July 2007

13 July 2007

Week 4

16 July 2007

17 July 2007

18 July 2007

19 July 2007

20 July 2007

Week 5

23 July 2007

24 July 2007

25 July 2007

26 July 2007

27 July 2007

Week 6

30 July 2007

31 July 2007

1 Aug 2007

2 Aug 2007

3 Aug 2007