Melbourne/Lab Notebook Weeks 13-16

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Contents

Week 13

16 Sept 2007


17 Sept 2007

Gel1:

    1. DNA ladder
    2. P17OA E/H
    3. P17OB E/H
    4. P1 5G A E/H
    5. P1 5G B E/H
    6. Omp-C1-reporter A X/P
    7. Omp-C1-reporter B X/P
    8. Omp-C1-reporter C X/P
    9. Omp-C1-reporter D X/P
    10. P2 21A A X/P
    11. P2 21A B X/P
    12. P2 21C A X/P
    13. P2 21C B X/P
    14. Death-ComA 5min ligation A X/P
    15. Death-ComA 5min ligation B X/P
    16. Death-ComA overnight ligation A X/P
    17. Death-ComA overnight ligation B X/P
    18. DNA ladder

Gel2:

    1. DNA ladder
    2. Death-ComP A X/P
    3. Death-ComP B X/P
    4. Cph8 A X/P
    5. Cph8 B X/P


-All had desired insert except for lanes 8-11 (OmpR-C1-reporter) in Gel 1 and lanes 4, 5 (CpH8) in Gel 2.


18 Sept 2007

  • Liquid cultured 4 colonies each from transformants of 17/9 (2 from Death-ComA).


19 Sept 2007

-Ran at 100V for 45min. -Lanes 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, and 15 had desired bands.



20 Sept 2007


21 Sept 2007


22 Sept 2007

Week 14

23 Sept 2007


24 Sept 2007


25 Sept 2007


26 Sept 2007

  • Miniprepped the following
    • TetR-RBS-P2,21A-ter A,B,C and D
    • TetR-RBS-ComP-ter A,B,C and D
    • GenRBS-ComA-ter A,B,C and D
    • cI-L3 A,B,C and D

27 Sept 2007


28 Sept 2007


29 Sept 2007


Week 15

30 Sept 2007


1 Oct 2007


2 Oct 2007


3 Oct 2007


4 Oct 2007


5 Oct 2007


6 Oct 2007


Week 16

7 Oct 2007


8 Oct 2007


9 Oct 2007


10 Oct 2007


11 Oct 2007


12 Oct 2007


13 Oct 2007