Melbourne/Miniprep protocol
From 2007.igem.org
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=====Method including controls===== | =====Method including controls===== | ||
| - | + | * Use Protocol included in the Kit. | |
| - | + | *A centrifugation method is used. | |
| - | + | *Modifications: | |
| + | In an effort to increase the yield of DNA, the following can be performed. | ||
| + | **The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul. | ||
| + | **The volume of bacterial culture used can be increased and centrifuged in two or more spins. | ||
| + | |||
=====Equipement Required===== | =====Equipement Required===== | ||
| - | * | + | *[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]] |
*Pipettes and tips | *Pipettes and tips | ||
*Microfuge | *Microfuge | ||
Revision as of 12:14, 28 September 2007
<Return to list of protocols> <Team home page>
- Applications:
- Isolation of plasmid DNA from Bacterial cells
- Time to complete protocol:
- Lab time: 1.5hrs
- Waiting time: 10min
- Approximate cost of materials: $0.00
Method from primary and secondary reagents
Primary & secondary Reagents Required including controls
- Miniprep kit
- TAE buffer if DNA intended for long term storage
Method including controls
- Use Protocol included in the Kit.
- A centrifugation method is used.
- Modifications:
In an effort to increase the yield of DNA, the following can be performed.
- The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
- The volume of bacterial culture used can be increased and centrifuged in two or more spins.
Equipement Required
- microcentrifuge: 1.7ml
- Pipettes and tips
- Microfuge
References
- Patricia Illing 03:49, 3 July 2007 (EDT):Miniprep protocol 3/July/2007
