Melbourne/Miniprep protocol
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=====Primary & secondary Reagents Required including controls===== | =====Primary & secondary Reagents Required including controls===== | ||
*[[Melb:Miniprep kit|Miniprep kit]] | *[[Melb:Miniprep kit|Miniprep kit]] | ||
| - | *TAE | + | *[[Melbourne/Secondary Reagent TAE|TAE Buffer]] if DNA intended for long term storage |
=====Method including controls===== | =====Method including controls===== | ||
Latest revision as of 11:08, 29 September 2007
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- Applications:
- Isolation of plasmid DNA from Bacterial cells
- Time to complete protocol:
- Lab time: 1.5hrs
- Waiting time: 10min
- Approximate cost of materials: $0.00
Method from primary and secondary reagents
Primary & secondary Reagents Required including controls
- Miniprep kit
- TAE Buffer if DNA intended for long term storage
Method including controls
- Use Protocol included in the Kit.
- A centrifugation method is used.
- Modifications:
In an effort to increase the yield of DNA, the following can be performed.
- The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
- The volume of bacterial culture used can be increased and centrifuged in two or more spins.
Equipement Required
- microcentrifuge: 1.7ml
- Pipettes and tips
- Microfuge
References
- Patricia Illing 03:49, 3 July 2007 (EDT):Miniprep protocol 3/July/2007
