Melbourne/Miniprep protocol

From 2007.igem.org

< Melbourne(Difference between revisions)
(Primary & secondary Reagents Required including controls)
 
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=====Primary & secondary Reagents Required including controls=====
=====Primary & secondary Reagents Required including controls=====
*[[Melb:Miniprep kit|Miniprep kit]]
*[[Melb:Miniprep kit|Miniprep kit]]
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*TAE buffer if DNA intended for long term storage
+
*[[Melbourne/Secondary Reagent TAE|TAE Buffer]] if DNA intended for long term storage
=====Method including controls=====
=====Method including controls=====
-
#
+
* Use Protocol included in the Kit.
-
#
+
*A centrifugation method is used.
-
#
+
*Modifications:
 +
In an effort to increase the yield of DNA, the following can be performed.
 +
**The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
 +
**The volume of bacterial culture used can be increased and centrifuged in two or more spins.
 +
 
=====Equipement Required=====
=====Equipement Required=====
-
*Microfuge tubes
+
*[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]]
*Pipettes and tips
*Pipettes and tips
*Microfuge
*Microfuge

Latest revision as of 11:08, 29 September 2007

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  • Applications:
    1. Isolation of plasmid DNA from Bacterial cells
  • Time to complete protocol:
    • Lab time: 1.5hrs
    • Waiting time: 10min
  • Approximate cost of materials: $0.00

Method from primary and secondary reagents

Primary & secondary Reagents Required including controls
Method including controls
  • Use Protocol included in the Kit.
  • A centrifugation method is used.
  • Modifications:

In an effort to increase the yield of DNA, the following can be performed.

    • The final elution can be repeated. The first elution is done using 50ul of Nuclease-Free Water and the second using 30ul.
    • The volume of bacterial culture used can be increased and centrifuged in two or more spins.
Equipement Required
References