Melbourne/Plan/Red Photosensor

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[[Melb:Background|<return to top of background>]]  [[melbourne|<return to home page>]]    [[Melb:Blue Photosensor|<next>]]
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[[Melbourne/Plan|<return to top of plan>]]  [[melbourne|<return to home page>]]    [[Melb:Blue Photosensor|<next>]]
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This component is used to detect one of the light sources 660nm filter. It was developed at MIT and used by the coliroid team 2005.
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The Plan for the implementation of the blue light system into a functioning BioBrick
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* D - Day
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*It is a standard component: (EnvZ-OmpR) BBaI15010  **
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* N - Night
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*But requires biosynthetic parts: (ho1)   BBa15008
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* (pcyA) BBa15009
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== Timeline ==
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*Output is via positively regulating promoter:   (ompC) BBaR0082
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Week 0 (week beginning 29/07/07)
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* ligations ...  
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Characterisation Here
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* craig starting to make test harness
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Consider usefulness of BBaM30109?? (4.3Kbp)
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Week 1 (week beginning 06/08/07)
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REQUIRE A ENZ DELETED BACTERIAL STRAIN.
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* D1: Ligate Gen-RBS into LacZ
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* D2: Transform onto +Gen,+Kan plates
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* D3: Pick colonies and culture
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* D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
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* N4: Ligate. We might be able to do a short ligation during the day.
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* D5: Transform onto +Gen, +Kan plates
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* D6: Pick colonies and culture
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Week 2 (week beginning 06/08/07)
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* D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
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* N8: Ligate
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* D9: Transform onto +Amp plates
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* D10: Pick colonies
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* D11: Miniprep
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* D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
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* D13: Transform into EnvZ-
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* D14: Pick colonies
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Week 3 (week beginning 13/08/07)
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Transformation into EnvZ-
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* D1: Make cells competent
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* D2: Transform cells with construct.
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Week 4 (week beginning 20/08/07)
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Week 5 (week beginning 27/08/07)
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* Rinse and repeat

Latest revision as of 23:38, 6 August 2007

<return to top of plan> <return to home page> <next>

The Plan for the implementation of the blue light system into a functioning BioBrick

  • D - Day
  • N - Night

Timeline

Week 0 (week beginning 29/07/07)

  • ligations ...
  • craig starting to make test harness


Week 1 (week beginning 06/08/07)

  • D1: Ligate Gen-RBS into LacZ
  • D2: Transform onto +Gen,+Kan plates
  • D3: Pick colonies and culture
  • D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
  • N4: Ligate. We might be able to do a short ligation during the day.
  • D5: Transform onto +Gen, +Kan plates
  • D6: Pick colonies and culture


Week 2 (week beginning 06/08/07)

  • D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
  • N8: Ligate
  • D9: Transform onto +Amp plates
  • D10: Pick colonies
  • D11: Miniprep
  • D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
  • D13: Transform into EnvZ-
  • D14: Pick colonies


Week 3 (week beginning 13/08/07)

Transformation into EnvZ-

  • D1: Make cells competent
  • D2: Transform cells with construct.

Week 4 (week beginning 20/08/07)


Week 5 (week beginning 27/08/07)

  • Rinse and repeat
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