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Revision as of 12:27, 6 August 2007 (view source) Honza83 (Talk | contribs) ← Older edit		Revision as of 23:37, 6 August 2007 (view source) Honza83 (Talk | contribs) Newer edit →
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	Week 1 (week beginning 06/08/07)		Week 1 (week beginning 06/08/07)
-	* sequence comes in !!!	+
-	* screen	+	* D1: Ligate Gen-RBS into LacZ
-	* plate (DN)	+	* D2: Transform onto +Gen,+Kan plates
-	* culture (DN)	+	* D3: Pick colonies and culture
-	* miniprep & digest & gel (D)	+	* D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
-	* ligate (N)	+	* N4: Ligate. We might be able to do a short ligation during the day.
-	* transform into DH5-alpha (DN)	+	* D5: Transform onto +Gen, +Kan plates
-	* screen (colony PCR) (D)	+	* D6: Pick colonies and culture
-	* culture (N)	+
		+
		+	Transformation into EnvZ-
		+
		+
	Week 2 (week beginning 06/08/07)		Week 2 (week beginning 06/08/07)
-	* digest, purify (D)
-	* ligate (N)
-	* transform (DN)
-	* screen (D)
-	***** need to expand on this
		+	* D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
		+	* N8: Ligate
		+	* D9: Transform onto +Amp plates
		+	* D10: Pick colonies
		+	* D11: Miniprep
		+	* D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
		+	* D13: Transform into EnvZ-
		+	* D14: Pick colonies
	Week 3 (week beginning 13/08/07)		Week 3 (week beginning 13/08/07)
		+
		+	* D1: Make cells competent
		+	* D2: Transform cells with construct.
	Week 4 (week beginning 20/08/07)		Week 4 (week beginning 20/08/07)

---

Revision as of 23:37, 6 August 2007

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The Plan for the implementation of the blue light system into a functioning BioBrick

• D - Day
• N - Night

Timeline

Week 0 (week beginning 29/07/07)

• ligations ...
• craig starting to make test harness

Week 1 (week beginning 06/08/07)

• D1: Ligate Gen-RBS into LacZ
• D2: Transform onto +Gen,+Kan plates
• D3: Pick colonies and culture
• D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
• N4: Ligate. We might be able to do a short ligation during the day.
• D5: Transform onto +Gen, +Kan plates
• D6: Pick colonies and culture

Transformation into EnvZ-

Week 2 (week beginning 06/08/07)

• D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
• N8: Ligate
• D9: Transform onto +Amp plates
• D10: Pick colonies
• D11: Miniprep
• D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
• D13: Transform into EnvZ-
• D14: Pick colonies

Week 3 (week beginning 13/08/07)

• D1: Make cells competent
• D2: Transform cells with construct.

Week 4 (week beginning 20/08/07)

Week 5 (week beginning 27/08/07)

• Rinse and repeat