Melbourne/Plan/Red Photosensor

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The Plan for the implementation of the blue light system into a functioning BioBrick

  • D - Day
  • N - Night

Timeline

Week 0 (week beginning 29/07/07)

  • ligations ...
  • craig starting to make test harness


Week 1 (week beginning 06/08/07)

  • D1: Ligate Gen-RBS into LacZ
  • D2: Transform onto +Gen,+Kan plates
  • D3: Pick colonies and culture
  • D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
  • N4: Ligate. We might be able to do a short ligation during the day.
  • D5: Transform onto +Gen, +Kan plates
  • D6: Pick colonies and culture


Week 2 (week beginning 06/08/07)

  • D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
  • N8: Ligate
  • D9: Transform onto +Amp plates
  • D10: Pick colonies
  • D11: Miniprep
  • D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
  • D13: Transform into EnvZ-
  • D14: Pick colonies


Week 3 (week beginning 13/08/07)

Transformation into EnvZ-

  • D1: Make cells competent
  • D2: Transform cells with construct.

Week 4 (week beginning 20/08/07)


Week 5 (week beginning 27/08/07)

  • Rinse and repeat