Melbourne/Primary Reagents & disposables
From 2007.igem.org
(Difference between revisions)
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==Primary Reagents== | ==Primary Reagents== | ||
===Kits=== | ===Kits=== | ||
| - | *Miniprep kit | + | *[[Melb:Miniprep kit|Miniprep kit]] |
*Gel clean-up system | *Gel clean-up system | ||
*Site directed mutagenesis (stratagene) | *Site directed mutagenesis (stratagene) | ||
Revision as of 05:36, 3 July 2007
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Contents |
Primary Reagents
Kits
- Miniprep kit
- Gel clean-up system
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Enzymes
- Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
- Ligase
- Competent cells (both for plasmid purification and protein expression)
- DNA standard
Chemicals
- Ingredients for media (LB, SOB)
- Glycerol
- Buffers (including TAE and TE)
- Loading dye for DNA gel
Disposables
TUBES:
- Falcon: 10ml, 50ml
- microcentrifuge: 1.7ml
- PCR
Tips
- Tips (10, 200, 1000 ul)
Other
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
- Parafilm
