Melbourne/Secondary Reagent LB

From 2007.igem.org

(Difference between revisions)
(Method:)
Line 9: Line 9:
For 1L:
For 1L:
-
*10g bacto-tryptone
+
*10g [[Melbourne/primary Bacto-tryptone|Bacto-tryptone]]
-
*5g yeast extract
+
*5g [[Melbourne/primary Yeast Extract|Yeast Extract]]
-
*10g NaCl
+
*10g[[Melbourne/primary Nacl|NaCl]]
-
*NaOH to adjust pH
+
*[[Melbourne/primary NaOH|NaOH]] to adjust pH
===Method:===
===Method:===
#Place Ingredients in a plastic container and add sterile water to the desired volume.  
#Place Ingredients in a plastic container and add sterile water to the desired volume.  
#Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.
#Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.

Revision as of 11:02, 29 September 2007

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Contents

Preparation of LB

Uses

Ingredients:

For 1L:

Method:

  1. Place Ingredients in a plastic container and add sterile water to the desired volume.
  2. Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.