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Revision as of 08:49, 29 September 2007 (view source) Craig (Talk | contribs) ← Older edit		Latest revision as of 11:05, 29 September 2007 (view source) Craig (Talk | contribs) (→Ingedients:)
Line 8:		Line 8:
	Per Liter:		Per Liter:
-	*10g NZ amine	+	*10g [[Melbourne/primary NZ amine|NZ amine]]
	*5g [[Melbourne/primary Nacl|NaCl]]		*5g [[Melbourne/primary Nacl|NaCl]]
-	*5g Bacto-yeast extract	+	*5g [[Melbourne/primary Bacto-yeast Extract|Bacto-yeast Extract]]
-	*NaOH to adjust pH	+	*[[Melbourne/primary NaOH|NaOH]] to adjust pH
	*Sterile water		*Sterile water

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Latest revision as of 11:05, 29 September 2007

<return to list of secondary methods> <team home page>

Contents 1 Preparation of NZY media 2 Uses 3 Ingedients: 4 Method: 5 Equipment:

Preparation of NZY media

Uses

• Site-directed mutagenesis

Ingedients:

Per Liter:

• 10g NZ amine
• 5g NaCl
• 5g Bacto-yeast Extract
• NaOH to adjust pH
• Sterile water

Method:

1. Mix ingredients in Sterile water using a magnetic stirrer
2. Adjust pH to 7.5 using NaOH.

Equipment:

Magnetic Stirrer