Melbourne/Secondary Reagent NYZ

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Contents

Preparation of NZY media

Uses

  • Site-directed mutagenesis

Ingedients:

Per Liter:

  • 10g NZ amine
  • 5g NaCl
  • 5g Bacto-yeast extract
  • NaOH to adjust pH
  • Sterile water

Method:

  1. Mix ingredients in Sterile water using a magnetic stirrer
  2. Adjust pH to 7.5 using NaOH.

Equipment:

Magnetic Stirrer

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