Melbourne/primary vf2

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*Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert
*Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert
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*Primers were designed identical to the sites on the Registry
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====Primer design and maintenance====
 +
*Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry.
*Primers were ordered and purchased from Sigma
*Primers were ordered and purchased from Sigma
*We keep a working concentration of 10uM and a stock solution of 1mM.
*We keep a working concentration of 10uM and a stock solution of 1mM.
 +
====Problems====
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*Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use.
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*See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details.

Latest revision as of 01:50, 27 October 2007

<return to list of primary reagents> <team home page>

Contents

Physical data

  • Description: Forward primer for backbone vector
  • Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert

Primer design and maintenance

  • Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry.
  • Primers were ordered and purchased from Sigma
  • We keep a working concentration of 10uM and a stock solution of 1mM.


Problems

  • Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use.
  • See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details.


Lab Location


Manufacturer:

  • Manufacturer:Sigma
  • Manufacturers