Mexican Wave

From 2007.igem.org

(Difference between revisions)
(Project 1 Mexican Wave)
(Project 1 Mexican Wave)
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=Project 1    Mexican Wave =
=Project 1    Mexican Wave =
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FIRST VIEW
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'''FIRST VIEW'''
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*  Our first thought was to create a pattern by the change of colors in a concentration dependent manner; so that the production of a protein at the end of a different event would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.  
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*  Our first thought was to create a pattern by the change of colors (by production of different fluorescent proteins) triggered by a signalling protein in a concentration dependent manner; so that the production of a protein as a result of the processing of a separete system, would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.  
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* The plan was to have a plate having a lawn of same type E. coli cells having 3 constructs. Each construct would have a promoter activated by a different level of the initial signal protein and a reporter fluorescent protein coding part.
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*   The triggering protein A is being dropped on the middle of the plate, creating a concentration gradient on the plate as in the figure. Acording to this plan the color change will be once, and then by the rising level of [A], the plate will all become green.
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* The idea was to have a plate having a lawn of cloned E. coli cells having 3 constructs. Each construct would have a promoter, which would be activated by a different level of the initial signal protein, and a coding part for a reporter fluorescent protein.
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* The triggering protein (say protein A) would be dropped at the middle of the plate, creating a concentration gradient on the plate as it diffuses. According to this, the color change will be for once, and then by the rising level of [A], the plate will all become green.
[[Image:taslak.jpg]]
[[Image:taslak.jpg]]
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SECOND VIEW
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'''SECOND VIEW'''
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*  The second view was to make this color change continuous and independent of a created concentration gradient. So first idea came up to be coupling this oscillation to an already oscillating protein, maybe to cell cycle. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of different cells on the plate.
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*  The second view was to make this color change continuous and independent of a concentration gradient gradient created by us. So first idea came up to be coupling this oscillation to an already oscillating protein in the cell, such as a cell cycle protein. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of different cells on the plate.

Revision as of 03:06, 27 October 2007

Project 1 Mexican Wave

FIRST VIEW

  • Our first thought was to create a pattern by the change of colors (by production of different fluorescent proteins) triggered by a signalling protein in a concentration dependent manner; so that the production of a protein as a result of the processing of a separete system, would trigger the pattern formation, just like a goal triggering a *Mexican wave in soccer games.
  • The idea was to have a plate having a lawn of cloned E. coli cells having 3 constructs. Each construct would have a promoter, which would be activated by a different level of the initial signal protein, and a coding part for a reporter fluorescent protein.
  • The triggering protein (say protein A) would be dropped at the middle of the plate, creating a concentration gradient on the plate as it diffuses. According to this, the color change will be for once, and then by the rising level of [A], the plate will all become green.

Taslak.jpg

SECOND VIEW

  • The second view was to make this color change continuous and independent of a concentration gradient gradient created by us. So first idea came up to be coupling this oscillation to an already oscillating protein in the cell, such as a cell cycle protein. But then this oscillation will be observable only on a single cell unless we synchronize the cell cycles of different cells on the plate.