NYMU Taipei/Available Restriction Enzymes

From 2007.igem.org

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<p><font size="3"><font color="#810081"><a href="http://www.krackeler.com/products/fid/2753">Unit Definition of Restriction Enzymes</a></font></font></p>
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<p><font size="3"><font color="#810081">[http://www.krackeler.com/products/fid/2753 Unit Definition of Restriction Enzymes]</font></font></p>
<ul>
<ul>
     <li><font size="2">One unit is defined as the amount of enzyme required to hydrolyze 1&micro;g of &lambda; DNA to completion in 60 minutes in a total reaction volume of 50 &micro;l, under optimal assay conditions (salt concentration, pH, temperature). </font></li>
     <li><font size="2">One unit is defined as the amount of enzyme required to hydrolyze 1&micro;g of &lambda; DNA to completion in 60 minutes in a total reaction volume of 50 &micro;l, under optimal assay conditions (salt concentration, pH, temperature). </font></li>
     <li><font size="2">Some enzymes which do not, or rarely, cleave &lambda; DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.</font></li>
     <li><font size="2">Some enzymes which do not, or rarely, cleave &lambda; DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.</font></li>
</ul>
</ul>
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<p><font size="3"><a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp">Setting Up a Restriction Endonuclease Reaction</a></font></p>
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<p><font size="3">[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp Setting Up a Restriction Endonuclease Reaction]</font></p>
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<p><font size="3"><a href="http://www.promega.com/guides/re_guide/chaptwo/2_1.htm">Standard Restriction Enzyme Reactions</a></font></p>
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<p><font size="3">[http://www.promega.com/guides/re_guide/chaptwo/2_1.htm Standard Restriction Enzyme Reactions]</font></p>
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<p><font size="3"><a href="http://www.neb.com/nebecomm/products/faqcategory1.asp">FAQs for Restriction Endonucleases</a></font></p>
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<p><font size="3">[http://www.neb.com/nebecomm/products/faqcategory1.asp FAQs for Restriction Endonucleases]</font></p>
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<p><font size="3"><a href="http://www.ndsu.edu/instruct/mcclean/plsc731/dna/dna5.htm">Restriction Modification System</a></font></p>
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<p><font size="3">[http://www.ndsu.edu/instruct/mcclean/plsc731/dna/dna5.htm Restriction Modification System]</font></p>
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<p><font size="3"><a href="http://www.everythingbio.com/glos/definition.php?word=star+activity">Definition of star activity</a></font></p>
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<p><font size="3">[ttp://www.everythingbio.com/glos/definition.php?word=star+activity Definition of star activity]</font></p>
<ul>
<ul>
     <li><font size="2">AatII </font></li>
     <li><font size="2">AatII </font></li>

Revision as of 13:13, 18 October 2007

Unit Definition of Restriction Enzymes

  • One unit is defined as the amount of enzyme required to hydrolyze 1µg of λ DNA to completion in 60 minutes in a total reaction volume of 50 µl, under optimal assay conditions (salt concentration, pH, temperature).
  • Some enzymes which do not, or rarely, cleave λ DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.

Setting Up a Restriction Endonuclease Reaction

Standard Restriction Enzyme Reactions

FAQs for Restriction Endonucleases

Restriction Modification System

[ttp://www.everythingbio.com/glos/definition.php?word=star+activity Definition of star activity]

  • AatII
  • AccI
  • AciI
  • AseI
  • BamHI
  • BanII
  • BbsI
  • BbvI
  • BglII
  • BsaBI
  • BspEI
  • EcoRI
  • EcoRV
  • HindIII
  • MboI
  • MspI
  • NcoI
  • NdeI
  • NheI
  • NotI
  • PacI
  • PstI
  • RsaI
  • SacI
  • SalI
  • SmaI
  • SpeI
  • SwaI
  • XbaI
  • XhoI
  • XmaI
  • XmnI

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