NYMU Taipei/Available Restriction Enzymes

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Unit Definition of Restriction Enzymes

  • One unit is defined as the amount of enzyme required to hydrolyze 1µg of λ DNA to completion in 60 minutes in a total reaction volume of 50 µl, under optimal assay conditions (salt concentration, pH, temperature).
  • Some enzymes which do not, or rarely, cleave λ DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.

Setting Up a Restriction Endonuclease Reaction

Standard Restriction Enzyme Reactions

FAQs for Restriction Endonucleases

Restriction Modification System

Definition of star activity

  • AatII
  • AccI
  • AciI
  • AseI
  • BamHI
  • BanII
  • BbsI
  • BbvI
  • BglII
  • BsaBI
  • BspEI
  • EcoRI
  • EcoRV
  • HindIII
  • MboI
  • MspI
  • NcoI
  • NdeI
  • NheI
  • NotI
  • PacI
  • PstI
  • RsaI
  • SacI
  • SalI
  • SmaI
  • SpeI
  • SwaI
  • XbaI
  • XhoI
  • XmaI
  • XmnI

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