NYMU Taipei/Help me!

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Contents

Q1: Infrared Emitting Protein

  • I've found parts that cantranslate green, yellow, and red fluorescence protein, but I can't find that can emit infrared rays. Can anyone help me find it? (posted by De-Ming Lin)
    • I am not sure there is a one can emit infrared light.
  • What is your original idea for having the special part?
    • If we can combine glucose promoter and structural gene which can translate protein that can emit infrared ray, and put it on the finger tip, we can measure blood sugar by an non-invasive way.
    • I think it is the question about how to measure the promoter activity under glucose stimulus in our system (8/30).

Q2: Documentation of Projects

  • For documentation, please all create a page for each of the five candidate projects on "My hot teams" and describe your design there. Uploading all the files of powerpoint or reference literature is very welcome! Thank you all very much for efforts of documentation ^^ Can we just document the two selected projects?
    • Reply: I just want to make the "brainstorming" part of our documentation as lush as we can ^^" I know everybody is busy, so maybe some description words with some files already done (powerpoint and literature showed on 7/13) are plenty enough ^^
  • Should we have a good framework about how to record our experiments and ideas? (8/30)

Q3: Problems about Materials and Designs

Q4: how to read the plate in iGEM 2007 Kit?

Q5: Detail sequence information needed for the parts in our design

Q6: Requests for chemicals

Q7: How to detect the glucose concentration in our project?

Q8: How to participate our wet lab works?

  • Assembly procedure for two parts
    • STEP 1: digest full plasmid into part DNA and vector DNA (2.0 h waiting, 0.5 h operation; total 2.5 h)
    • STEP 2: gel separation for part DNA and vector DNA (1.0 h waiting, 0.5 h operation; total 1.5 h)
    • STEP 3: ligation for 2 part DNAs (3.0 h waiting, 0.5 h operation; total 3.5 h)
    • STEP 4: transformation in plate culture (16.0 h waiting, 0.5 h operation; total 16.5 h)
    • STEP 5: liquid culture to amplify the combined part DNA (16.0 h waiting, 0.0 h operation; total 16.0 h), back to STEP 1 and repeat

Q9: We need an "available restriction enzymes list and an almost depleted reagents page.

Q10: How about the hotels for Jamboree 2007?

  • Holiday Inn: Boston Government Center
    • Price: $179US plus applicable tax
    • Transportation to/from: 15-20 minute scenic walk across the Charles river to the Jamboree or 2 minute walk to Charles/MGH T stop (<a class="external text" title="http://www.mbta.com" rel="nofollow" _toggle_original_display="" href="http://www.mbta.com/">MBTA</a>) on the Red line (take to Kendall Square to get to Jamboree)
  • Holiday Inn: Somerville
    • Price: $139US plus applicable tax
    • Transportation to/from:

Q11: About apply the US visa

Q12: For boys

 

 

Q13: Documentation

<a href="https://2007.igem.org/Jamboree/Compete#Online_Judging">https://2007.igem.org/Jamboree/Compete#Online_Judging</a>

    • Project Documentation on the iGEM Wiki

  • Description: The team's project should be documented on the iGEM 2007 wiki site with detail enough to replicate it independently. Additional presentational information about the team - their story, the rationale for the project, failures, successes, future work, etc. - is highly encouraged. Remember that these wiki pages will be the main source of inspiration for future teams, and having good documentation on them and in the part description in the registry increases the likelihood of more teams building on your project and your parts.
  • deadline: the iGEM wiki freezes 04:59 GMT Oct 27

    • Part Documentation on the Registry

  • Description: Document your parts well - the success of not just iGEM, but all of synthetic biology, depends on the development of well-characterized, reliable, standardized biological parts that have been designed to be simple to use and understand. The first step is complete documentation.
  • deadline: the Registry freezes 04:59 GMT Oct 27

 

Plan to document:

  • Regular meeting
  • My hot teams
  • Team schedule
  • Help me!
  • Protocols
  • Materials
  • Benchman
  • Our Part Registry 
  • Key experiments
  • Construct
  • Almost Depleted Reagents
  • You must know!
  • Paper

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