NYMU Taipei/Lab Notes/2007 10 2
From 2007.igem.org
(Difference between revisions)
(→re-culture pOmpC and pCinRHSL) |
(→digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term) |
||
| Line 12: | Line 12: | ||
** lane 18: OmpRBS (PCR 6) | ** lane 18: OmpRBS (PCR 6) | ||
== re-culture pOmpC and pCinRHSL == | == re-culture pOmpC and pCinRHSL == | ||
| - | == | + | == digestion for assembly == |
| + | === System 1 === | ||
| + | * pOmpC + TATA_INSA | ||
| + | ** pOmpC | ||
| + | ** TATA_INSA | ||
| + | * TATB_INSB + CinR+HSL+D-term | ||
| + | ** TATB_INSB | ||
| + | ** CinR+HSL+D-term | ||
| + | === System 2 === | ||
| + | * pCinRHSL + OmpRBS | ||
| + | ** pCinRHSL | ||
| + | ** OmpRBS | ||
| + | * (pCinRHSL + OmpRBS) + TATD_IDE | ||
| + | ** (pCinRHSL + OmpRBS) | ||
| + | ** TATD_IDE | ||
| + | * [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term | ||
| + | ** [(pCinRHSL + OmpRBS) + TATD_IDE] | ||
| + | ** D-term | ||
Revision as of 06:22, 3 October 2007
Contents |
TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR
- from left to right
- lane 1: 100bp ladder
- lane 2-4: TATA_INSA (PCR 1+2)
- lane 5-7: TATB_INSB (PCR 3+4)
- lane 8-12: OmpRBS (PCR 6)
- lane 13: 1kb ladder
- lane 14-16: TATD_IDE (PCR 5+7)
- lane 17: 100bp ladder
- lane 18: OmpRBS (PCR 6)
re-culture pOmpC and pCinRHSL
digestion for assembly
System 1
- pOmpC + TATA_INSA
- pOmpC
- TATA_INSA
- TATB_INSB + CinR+HSL+D-term
- TATB_INSB
- CinR+HSL+D-term
System 2
- pCinRHSL + OmpRBS
- pCinRHSL
- OmpRBS
- (pCinRHSL + OmpRBS) + TATD_IDE
- (pCinRHSL + OmpRBS)
- TATD_IDE
- [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
- [(pCinRHSL + OmpRBS) + TATD_IDE]
- D-term
