NYMU Taipei/Lab Notes/2007 10 2

From 2007.igem.org

TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR

NYMU Taipei TATA INSA INSB and OmpRBS GEL check.jpg NYMU Taipei TATD IDE and OmpRBS GEL check.jpg

  • from left to right
    • lane 1: 100bp ladder
    • lane 2-4: TATA_INSA (PCR 1+2)
    • lane 5-7: TATB_INSB (PCR 3+4)
    • lane 8-12: OmpRBS (PCR 6)
    • lane 13: 1kb ladder
    • lane 14-16: TATD_IDE (PCR 5+7)
    • lane 17: 100bp ladder
    • lane 18: OmpRBS (PCR 6)

re-culture pOmpC and pCinRHSL

digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term