NYMU Taipei/Lab Notes/2007 10 4

From 2007.igem.org

(Difference between revisions)
(digestion check of CinR+HSL+D-term,pCinRHSL and pOmpC (vectors))
(Re-check the concentration of inserts and vectors)
Line 25: Line 25:
* lane G: TATB_INSB
* lane G: TATB_INSB
* lane H: OmpRBS
* lane H: OmpRBS
 +
</td>
 +
<td>
 +
* owing to vectors
</td>
</td>
</tr>
</tr>
</table>
</table>

Revision as of 13:59, 4 October 2007

digestion check of CinR+HSL+D-term,pCinRHSL and pOmpC (vectors)

NYMU Taipei 20071004 CinR-HSL-D-term,pCinRHSL,pOmpC digestion check.jpg
  • lane A: 1kb ladder
  • lane B: CinR+HSL+D-term x 2
  • lane C: 1kb ladder
  • lane D: pCinRHSL x 2
  • lane E: pOmpC x 2
  • after gel separation, their O.D. was checked. However, it is too low

Re-check the concentration of inserts and vectors

NYMU Taipei 20071004 3 vectors 3 inserts digestion check.jpg
  • lane A: 1kb ladder
  • lane B: pCinRHSL
  • lane C: pOmpC
  • lane D: CinR+HSL+D-term
  • lane E: 100bp ladder
  • lane F: TATA_INSA
  • lane G: TATB_INSB
  • lane H: OmpRBS
  • owing to vectors