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NYMU Taipei/Lab Notes/2007 10 5 - Revision history
2024-03-28T13:06:38Z
Revision history for this page on the wiki
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Blent: /* PCR length check */
2007-10-09T05:17:18Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 05:17, 9 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* expected <ins class="diffchange diffchange-inline">combined insertion </ins>lengths of three ligations</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)</div></td></tr>
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Blent
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Blent: /* PCR length check */
2007-10-09T05:16:29Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 05:16, 9 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*** 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** <ins class="diffchange diffchange-inline"><s></ins>(III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145<ins class="diffchange diffchange-inline"></s></ins></div></td></tr>
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Blent
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Blent: /* PCR length check */
2007-10-09T05:16:10Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 05:16, 9 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*** 291 = pCinRHSL (225) + Sequence after left cutting sites (1) + OmpRBS (65)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*** 297 = pOmpC (108) + Sequence after left cutting sites (23) + TATA (90) + INSA (66)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145</div></td></tr>
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Blent
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Blent: /* PCR length check */
2007-10-05T08:34:15Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:34, 5 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): <ins class="diffchange diffchange-inline">291 + 316 = 613</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)<ins class="diffchange diffchange-inline">: 297 + 316 = 603</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)<ins class="diffchange diffchange-inline">: 1,829 + 316 = 2,145</ins></div></td></tr>
</table>
Blent
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Blent: /* PCR length check */
2007-10-05T08:21:59Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:21, 5 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert): </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</div></td></tr>
</table>
Blent
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Blent: /* PCR length check */
2007-10-05T08:20:15Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:20, 5 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* expected lengths of three ligations</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** (I) pCinRHSL (vector) + OmpRBS (insert)<ins class="diffchange diffchange-inline">: </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (II) pOmpC (vector) + TATA_INSA (insert)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</div></td></tr>
</table>
Blent
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Blent: /* PCR length check */
2007-10-05T08:19:55Z
<p><span class="autocomment">PCR length check</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:19, 5 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== PCR length check ==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* expected lengths of three ligations</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** (I) pCinRHSL (vector) + OmpRBS (insert)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** (II) pOmpC (vector) + TATA_INSA (insert)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)</ins></div></td></tr>
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Blent
http://2007.igem.org/wiki/index.php?title=NYMU_Taipei/Lab_Notes/2007_10_5&diff=23020&oldid=prev
Blent at 08:12, 5 October 2007
2007-10-05T08:12:59Z
<p></p>
<p><b>New page</b></p><div>== PCR length check ==</div>
Blent