NYMU Taipei/Lab Notes/2007 10 5

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PCR length check

PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
expected lengths of three ligations
- (I) pCinRHSL (vector) + OmpRBS (insert): 291 + 316 = 613
- (II) pOmpC (vector) + TATA_INSA (insert): 297 + 316 = 603
- (III) TATB_INSB (insert) + CinR+HSL+D-term (vector): 1,829 + 316 = 2,145

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