NYMU Taipei/Lab Notes/2007 8 24

From 2007.igem.org

< NYMU Taipei/Lab Notes(Difference between revisions)
(Plasmid check)
(Plasmid check)
 
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==Plasmid check==
==Plasmid check==
-
3ul DNA+1ul RE
+
3ul DNA (5ml LB E. coli, miniprep in 50ul EB)+1ul RE, final volume: 20ul<br>
BBa_R0082 psB1A2_pOmpC<br>
BBa_R0082 psB1A2_pOmpC<br>
BBa_I15017 EYFP<br>
BBa_I15017 EYFP<br>
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<p>
<p>
[[Image:NYMU_Taipei_gel_20070824_plasmid_check_1.jpg|300px]]
[[Image:NYMU_Taipei_gel_20070824_plasmid_check_1.jpg|300px]]
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*Well 1 OmpR EcoRI
+
*Well 1 pOmpC EcoRI
-
*Well 2 OmpR SpeI
+
*Well 2 pOmpC SpeI
-
*Well 3 OmpR XbaI, PstI
+
*Well 3 pOmpC XbaI, PstI
*Well 4 EYFP EcoRI
*Well 4 EYFP EcoRI
*Well 5 EYFP SpeI
*Well 5 EYFP SpeI

Latest revision as of 11:02, 24 August 2007

Plasmid check

3ul DNA (5ml LB E. coli, miniprep in 50ul EB)+1ul RE, final volume: 20ul
BBa_R0082 psB1A2_pOmpC
BBa_I15017 EYFP
BBa_I15016 ECFP
BBa_C0076 HSL
BBa_B0034 RBS
BBa_C0077 CinR
BBa_B0014 D-term
BBa_R0077 pCinRHSL RBS+
BBa_R0078 pCinRHSL

NYMU Taipei gel 20070824 plasmid check 1.jpg

  • Well 1 pOmpC EcoRI
  • Well 2 pOmpC SpeI
  • Well 3 pOmpC XbaI, PstI
  • Well 4 EYFP EcoRI
  • Well 5 EYFP SpeI
  • Well 6 EYFP XbaI, PstI
  • Well 7 100bp ladder
  • Well 8 ECFP EcoRI
  • Well 9 ECFP SpeI
  • Well 10 ECFP XbaI, PstI
  • Well 11 HSL EcoRI
  • Well 12 HSL SpeI

NYMU Taipei Gel 20070824 plasmid check 2.jpg

  • Well 1 HSL XbaI, PstI
  • Well 2 CinR EcoRI
  • Well 3 CinR SpeI
  • Well 4 CinR XbaI, PstI
  • Well 5 pCinRHSL EcoRI
  • Well 6 pCinRHSL SpeI
  • Well 7 100bp ladder
  • Well 8 pCinRHSL XbaI, PstI
  • Well 9 Empty
  • Well 10 pCinRHSL+RBS EcoRI
  • Well 11 pCinRHSL+RBS SpeI
  • Well 12 pCinRHSL+RBS XbaI, PstI

NYMU Taipei Gel 20070824 plasmid check 3.JPG

  • Well 1 Empty
  • Well 2 RBS EcoRI
  • Well 3 RBS SpeI
  • Well 4 RBS XP
  • Well 5 100bp ladder
  • Well 6 D-term EcoRI
  • Well 7 Leaky well
  • Well 8 D-term EcoRI
  • Well 9 D-term XbaI, PstI