NYMU Taipei/Lab Notes/2007 8 29

From 2007.igem.org

(Difference between revisions)
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*Plasmid extraction:
*Plasmid extraction:
-
**pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5
+
**pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
**Extract from 1.5ml LB medium to 20ul EB buffer
**Extract from 1.5ml LB medium to 20ul EB buffer
**Stored in -20°C
**Stored in -20°C

Revision as of 06:41, 30 August 2007

  • Plasmid extraction:
    • pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
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