NYMU Taipei/Lab Notes/2007 8 29
From 2007.igem.org
(Difference between revisions)
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**Excitation: 514nm | **Excitation: 514nm | ||
**Emition: 527nm | **Emition: 527nm | ||
+ | *Liquid culture for MGC clones | ||
+ | ** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) | ||
+ | ** PUM1, AKAP1 for m.t. experiment |
Revision as of 06:56, 30 August 2007
- Plasmid extraction:
- pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
- Extract from 1.5ml LB medium to 20ul EB buffer
- Stored in -20°C
- EYFP spectral characteristics:
- Excitation: 514nm
- Emition: 527nm
- Liquid culture for MGC clones
- INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse)
- PUM1, AKAP1 for m.t. experiment