NYMU Taipei/Lab Notes/2007 8 29

From 2007.igem.org

(Difference between revisions)
Line 6: Line 6:
**Excitation: 514nm
**Excitation: 514nm
**Emition: 527nm
**Emition: 527nm
 +
*Liquid culture for MGC clones
 +
** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse)
 +
** PUM1, AKAP1 for m.t. experiment

Revision as of 06:56, 30 August 2007

  • Plasmid extraction:
    • pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
  • Liquid culture for MGC clones
    • INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse)
    • PUM1, AKAP1 for m.t. experiment