NYMU Taipei/Lab Notes/2007 8 29

From 2007.igem.org

(Difference between revisions)
Line 7: Line 7:
**Emition: 527nm
**Emition: 527nm
*Liquid culture for MGC clones
*Liquid culture for MGC clones
-
** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project
+
** INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
-
** PUM1, AKAP1 for m.t. project
+
** PUM1 (human), AKAP1 (human) for m.t. project
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by 立翔
by 立翔

Revision as of 07:05, 30 August 2007

  • Plasmid extraction:
    • pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
  • Liquid culture for MGC clones
    • INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
    • PUM1 (human), AKAP1 (human) for m.t. project

by 立翔