NYMU Taipei/Lab Notes/2007 8 30

From 2007.igem.org

(Difference between revisions)
Line 29: Line 29:
**37°C, 2h
**37°C, 2h
**Heat inactivation: 65°C 20min
**Heat inactivation: 65°C 20min
 +
*Plasmid extraction: H_IGFBP3, H_IGFBP7, H_INS, M_IDE
 +
**From 5ml LB medium to 50ul EB

Revision as of 06:36, 30 August 2007

  • Enzyme Digestion (RBS)
    • RBS: 11ul
    • SpeI: 1ul
    • PstI: 1ul
    • 10x buffer: 2, 2ul
    • 10x BSA: 2ul
    • H2O: 3ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
  • Enzyme Digestion (cinR)
    • cinR: 11ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer: 3, 2ul
    • 10x BSA: 2ul
    • H2O: 3ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
  • Enzyme Digestion (HSL)
    • HSL: 4ul
    • XbaI: 1ul
    • PstI: 1ul
    • 10x buffer: 3, 2ul
    • 10x BSA: 2ul
    • H2O: 10ul
    • Total: 20ul
    • 37°C, 2h
    • Heat inactivation: 65°C 20min
  • Plasmid extraction: H_IGFBP3, H_IGFBP7, H_INS, M_IDE
    • From 5ml LB medium to 50ul EB