NYMU Taipei/Lab Notes/2007 8 9

From 2007.igem.org

(Difference between revisions)
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* After first transformation
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* Transformation results
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** Pick single colony
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**[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : <font color="red">Only 1 colony</font>
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***[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR  
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**[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : <font color="red">8 colonies</font>
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***[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription  
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*Pick single colony and incubate at LB plate
*Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells)
*Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells)
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)

Revision as of 13:13, 9 August 2007

  • Transformation results
    • [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : Only 1 colony
    • [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : 8 colonies
  • Pick single colony and incubate at LB plate
  • Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
    • Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
    • Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)