NYMU Taipei/Lab Notes/2007 8 9

From 2007.igem.org

  • Transformation results
    • [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : Only 1 colony
    • [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : 8 colonies
  • Pick single colony and incubate at LB plate
  • Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
    • Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
    • Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)