NYMU Taipei/Lab Notes/2007 9 1
From 2007.igem.org
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**Heat inactivation: 65°C, 20min | **Heat inactivation: 65°C, 20min | ||
*Check RBS+cinR 1 with BsaBI digestion | *Check RBS+cinR 1 with BsaBI digestion | ||
+ | **RBS+cinR 1: 3ul | ||
+ | **BsaBI: 1ul | ||
+ | **10x buffer 2: 2ul | ||
+ | **H2O: 14ul | ||
+ | **60°C dry bath, 1h |
Revision as of 08:25, 1 September 2007
- Plasmid extraction: RBS+cinR 1, RBS+HSL 1, RBS+HSL 2, RBS+HSL 3 in 50ul EB
- The concentration was measured with spectrophotometer at 260nm
- RBS+cinR 1: 8ug/ul
- RBS+HSL 1: 3ug/ul
- RBS+HSL 2: 8ug/ul
- RBS+HSL 3: 6ug/ul
- Enzyme digest RBS+cinR 1
- RBS+cinR 1: 14ul
- SpeI: 1ul
- PstI 1ul
- 10x buffer 2: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Enzyme digest RBS+HSL 2
- RBS+HSL 2: 14ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer 3: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Enzyme digest RBS+HSL 3
- RBS+HSL 3: 14ul
- XbaI: 1ul
- PstI: 1ul
- 10x buffer 3: 2ul
- 10x BSA: 2ul
- 37°C water bath, 2h
- Heat inactivation: 65°C, 20min
- Check RBS+cinR 1 with BsaBI digestion
- RBS+cinR 1: 3ul
- BsaBI: 1ul
- 10x buffer 2: 2ul
- H2O: 14ul
- 60°C dry bath, 1h