NYMU Taipei/Lab Notes/2007 9 10
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< NYMU Taipei/Lab Notes(Difference between revisions)
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| - | *Ligation(H_INS_A) | + | *Ligation A(H_INS_A) |
**H_INS_A1:2ul | **H_INS_A1:2ul | ||
**pOmpC:2ul | **pOmpC:2ul | ||
| - | **10X ligase:1 ul | + | **10X ligase:1 ul(YEA) |
| - | **10X buffer:1 ul | + | **10X buffer:1 ul(YEA) |
**ddH2O:4ul | **ddH2O:4ul | ||
**total: 10ul | **total: 10ul | ||
| - | *Ligation(H_INS_A) | + | *Ligation B(H_INS_A) |
**H_INS_A1:3ul | **H_INS_A1:3ul | ||
**pOmpC:2ul | **pOmpC:2ul | ||
| - | **10X ligase:1 ul | + | **10X ligase:1 ul (YEA) |
| - | **10X buffer:1 ul | + | **10X buffer:1 ul (YEA) |
**ddH2O:3ul | **ddH2O:3ul | ||
**total: 10ul | **total: 10ul | ||
| + | *ligation time: | ||
*Transformation | *Transformation | ||
**ligated DNA:2ul | **ligated DNA:2ul | ||
**compenent cell:100ul | **compenent cell:100ul | ||
*apply the cells on the agar plate | *apply the cells on the agar plate | ||
Latest revision as of 10:44, 10 September 2007
- Ligation A(H_INS_A)
- H_INS_A1:2ul
- pOmpC:2ul
- 10X ligase:1 ul(YEA)
- 10X buffer:1 ul(YEA)
- ddH2O:4ul
- total: 10ul
- Ligation B(H_INS_A)
- H_INS_A1:3ul
- pOmpC:2ul
- 10X ligase:1 ul (YEA)
- 10X buffer:1 ul (YEA)
- ddH2O:3ul
- total: 10ul
- ligation time:
- Transformation
- ligated DNA:2ul
- compenent cell:100ul
- apply the cells on the agar plate
