NYMU Taipei/Lab Notes/2007 9 11

From 2007.igem.org

(Difference between revisions)
Line 6: Line 6:
*** insert (3 uL) vs. vector (1 uL) at 09/10, 2007
*** insert (3 uL) vs. vector (1 uL) at 09/10, 2007
*** a trivial failure (absent of vector part) at 09/08, 2007
*** a trivial failure (absent of vector part) at 09/08, 2007
 +
** According to YEA ligation protocol, it requires
 +
*** ligation buffer A 1uL
 +
*** ligation buffer B 1uL
 +
*** vector 50 ng or 100 ng (proposed by pett)
 +
*** insert 300 ng
 +
*** ligase 1uL
 +
----
 +
*** total 10 uL

Revision as of 14:37, 11 September 2007

  • Fresh back
    • two different PCR (INS_A and INS_B) have been done by 海威 (9/5, 2007) and 惇茹 (9/8, 2007)
    • we had failed three times for construction of pOmpC + INS_A using 惇茹's INS_A and following three different ligation protocol
      • insert (1 uL) vs. vector (1 uL) at 09/09, 2007
      • insert (2 uL) vs. vector (1 uL) at 09/10, 2007
      • insert (3 uL) vs. vector (1 uL) at 09/10, 2007
      • a trivial failure (absent of vector part) at 09/08, 2007
    • According to YEA ligation protocol, it requires
      • ligation buffer A 1uL
      • ligation buffer B 1uL
      • vector 50 ng or 100 ng (proposed by pett)
      • insert 300 ng
      • ligase 1uL

      • total 10 uL