NYMU Taipei/Lab Notes/2007 9 16

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Revision as of 07:27, 16 September 2007 by Blent (Talk | contribs)
  • Enzyme digestion: CinR+HSL (with RBS) with EcoRI, SpeI
    • CinR+HSL (1~4): 14ul
      • 因為濃度未知, 所以用最大體積
    • EcoRI: 1ul (20,000 units/ml)
    • SpeI: 1ul (10,000 units/ml)
    • 10x EcoRI buffer: 2ul
    • Total: 20ul
  • Enzyme digestion: D-term with EcoRI
    • D-term: 11ul (損失因子10, 目標重量300ng)
      • 300(ng) * 10 = 3(ug) = 0.27 (ug/uL) * X(uL), X = 3/0.27 = 11.11111111
    • EcoRI: 1ul
    • 10x EcoRI buffer: 2ul
    • H2O: 6ul
    • Total: 20ul
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