NYMU Taipei/Lab Notes/2007 9 2

From 2007.igem.org

< NYMU Taipei/Lab Notes(Difference between revisions)
 
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*Ligation of (RBS+cinR+RBS+HSL): 4(?) colonies
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*Ligation of (RBS+cinR+RBS+HSL): 4(?) clones
*Ligation of (RBS+cinR ver. 2): multiple(?) colonies
*Ligation of (RBS+cinR ver. 2): multiple(?) colonies
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+
*Transfer each to 4 5ml LB liquid culture medium with 1 clone respectly.
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*Transfer each to 4 LB liquid culture media with 1 colony respectly.
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**(RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4
**(RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4
**(RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4
**(RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4
*Culture at 37C for 8 hours
*Culture at 37C for 8 hours
*Store at 4C
*Store at 4C
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*Rearrange of our parts in the box "IGEM 2007 parts"
*Rearrange of our parts in the box "IGEM 2007 parts"
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----
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==Who attend?==
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* 威,惇茹,穎駿,志賢

Latest revision as of 03:08, 5 September 2007

  • Ligation of (RBS+cinR+RBS+HSL): 4(?) clones
  • Ligation of (RBS+cinR ver. 2): multiple(?) colonies
  • Transfer each to 4 5ml LB liquid culture medium with 1 clone respectly.
    • (RBS+cinR+RBS+HSL)1,(RBS+cinR+RBS+HSL)2,(RBS+cinR+RBS+HSL)3,(RBS+cinR+RBS+HSL)4
    • (RBS+cinR ver. 2)1,(RBS+cinR ver. 2)2,(RBS+cinR ver. 2)3,(RBS+cinR ver. 2)4
  • Culture at 37C for 8 hours
  • Store at 4C
  • Rearrange of our parts in the box "IGEM 2007 parts"

Who attend?

  • 威,惇茹,穎駿,志賢