From 2007.igem.org

Revision as of 07:50, 9 September 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Revision as of 10:20, 10 September 2007 (view source) Lihsiangyen (Talk | contribs) Newer edit →
Line 1:		Line 1:
	*Ligation(H_INS_A)		*Ligation(H_INS_A)
-	**H_INS_A1:4ul	+	**H_INS_A1:1ul
		+	**pOmpC:2ul
	**10X ligase:1 ul		**10X ligase:1 ul
	**10X buffer:1 ul		**10X buffer:1 ul
-	**ddH2O:4ul	+	**ddH2O:5ul
	**total: 10ul		**total: 10ul
	*Transformation		*Transformation

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Revision as of 10:20, 10 September 2007

• Ligation(H_INS_A)
  • H_INS_A1:1ul
  • pOmpC:2ul
  • 10X ligase:1 ul
  • 10X buffer:1 ul
  • ddH2O:5ul
  • total: 10ul
• Transformation
  • ligated DNA:2ul
  • compenent cell:100ul
• apply the cells on the agar plate