NYMU Taipei/Lab Notes/2007 9 9

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< NYMU Taipei/Lab Notes(Difference between revisions)
 
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*Ligation(H_INS_A)  
*Ligation(H_INS_A)  
-
**H_INS_A1:4ul
+
**H_INS_A1:1ul
 +
**pOmpC:2ul
**10X ligase:1 ul  
**10X ligase:1 ul  
**10X buffer:1 ul  
**10X buffer:1 ul  
-
**ddH2O:4ul
+
**ddH2O:5ul
**total: 10ul
**total: 10ul
 +
*ligation time:1hr30min
*Transformation
*Transformation
**ligated DNA:2ul
**ligated DNA:2ul
**compenent cell:100ul
**compenent cell:100ul
*apply the cells on the agar plate
*apply the cells on the agar plate
 +
 +
*plate culture failed!
 +
*possibility:concentration of H_INS_A1 is not high enough

Latest revision as of 10:30, 10 September 2007

  • Ligation(H_INS_A)
    • H_INS_A1:1ul
    • pOmpC:2ul
    • 10X ligase:1 ul
    • 10X buffer:1 ul
    • ddH2O:5ul
    • total: 10ul
  • ligation time:1hr30min
  • Transformation
    • ligated DNA:2ul
    • compenent cell:100ul
  • apply the cells on the agar plate
  • plate culture failed!
  • possibility:concentration of H_INS_A1 is not high enough
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