NYMU Taipei/Part Assembly

From 2007.igem.org

(Difference between revisions)
Line 18: Line 18:
**Qiagene Miniprep Kit
**Qiagene Miniprep Kit
* Enzyme digestion to ligation formula
* Enzyme digestion to ligation formula
-
** [[NYMU Taipei/phptest phptest]]
+
**

Revision as of 10:17, 1 September 2007

  • Enzyme Digestion (2h)
    • Restriction enzymes: 1ul each
    • Incubate for 2h
    • Heat inactivation at 65°C, 20min
  • Gel Separation (0.5h)
    • 100V, 30min
  • Gel Extraction (0.5h)
    • Qiagene GelExtraction Kit
  • Ligation (3hr)
    • T4 ligase: 1ul
    • Incubate at RT for 3h
  • Plate Culture (16h)
    • DNA: 2ul
    • Competent cell: 50ul
  • Liquid Culture (16h)
    • 5ml LB
  • Plasmid Extraction (1hr)
    • Qiagene Miniprep Kit
  • Enzyme digestion to ligation formula