NYMU Taipei/Part List
From 2007.igem.org
(Difference between revisions)
(→antibiotics keys) |
(→antibiotics keys) |
||
Line 159: | Line 159: | ||
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin | ** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin | ||
** stock (100 mg/mL = 100 ug/uL) ampicilin | ** stock (100 mg/mL = 100 ug/uL) ampicilin | ||
- | ** liquid culture ( | + | ** liquid culture (50 ug/mL = 250 ug/5mL) |
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicilin | ** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicilin | ||
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin] | * K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin] |
Revision as of 06:49, 30 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
Part List
Series | alias | Location | Description | part length | vector (antibotics) | Check |
---|---|---|---|---|---|---|
BBa_R0082 | pOmpC | 1 | Positively regulated, OmpR-controlled promoter | 108 | pSB1A2 | ? |
BBa_C0082 | tar-envZ | 1 | Receptor, tar-envZ | 1491 | pSB2K3 | |
BBa_B0034 | RBS | 1,2,3 | The Elowitz RBS (Ribosome binding site) is the definition of efficiency 1.0 | 12 | pSB1A2 | ? |
AU43-C7 | INS | human insulin | 495 | C | ||
INS_A | 1 | alpha chain of human insulin | 63 | |||
INS_B | 1 | beta chain of human insulin | 90 | |||
BBa_B0014 | D-term | 1,2,3 | Double transcription terminator | 95 | pSB1AK3 | OK |
BBa_I14032 | pLacIQ | 2 | Constitutive Promoter, High Transcription | 37 | pSB2K3 | |
OmpRBS | 2 | OmpR binding sites | ? | |||
AV88-C6 | IDE | 2 | insulin degradation enzyme of mouse | 3445 | A | |
AU61-F6 | IGFBP3 | 2 | Insulin-like growth factor binding protein 3 of human | 2488 | C | |
AU50-C7 | IGFBP7 | 2 | Insulin-like growth factor binding protein 7 of human | 1155 | C | |
BBa_I15016 | B0032.ECFP | 16D, plate 1 | This part accepts POPS to produce ECFP under the medium RBS B0032 | 742 | pSB1A2 | OK |
BBa_I15017 | B0032.EYFP | 16F, plate 1 | This part accepts POPS to generate EYFP under the medium strength RBS B0032 | 742 | pSB1A2 | OK |
BBa_R0077 | pCinRHSL, RBS+ | 17L, Plate 1 | CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS. | 231 | pSB1A2 | OK |
BBa_R0078 | pCinRHSL | 15D, Plate 1 | CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS. | 225 | pSB1A2 | ? |
BBa_C0077 | CinR | 7G, Plate 2 | cinR activator | 762 | pSB2K3 | OK |
BBa_C0076 | HSL | 7E, Plate 2 | autoinducer | 702 | pSB2K3 | OK |
antibiotics keys
- A: Ampicillin
- refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
- stock (100 mg/mL = 100 ug/uL) ampicilin
- liquid culture (50 ug/mL = 250 ug/5mL)
- 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicilin
- K: Kanamycin
- C: Chloramphenicol
- 35 mg/mL
- 20 mg/mL in Ethanol
- we prepared 100mg in 5 mL and stored in -20 C