NYMU Taipei/Part List

From 2007.igem.org

(Difference between revisions)
(antibiotics keys)
(Part List)
 
(4 intermediate revisions not shown)
Line 79: Line 79:
|2
|2
|OmpR binding sites
|OmpR binding sites
-
|
+
|65
|
|
|?
|?
Line 117: Line 117:
|This part accepts POPS to generate EYFP under the medium strength RBS B0032
|This part accepts POPS to generate EYFP under the medium strength RBS B0032
|742
|742
 +
|pSB1A2
 +
|OK
 +
|-
 +
|[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]
 +
|B0030(RBS).E0040(GFP).B0015(D-term)
 +
|16E, plate 1
 +
|gfp tri-part; strong rbs
 +
|878
|pSB1A2
|pSB1A2
|OK
|OK
Line 158: Line 166:
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
-
** stock (100 mg/mL = 100 ug/uL) ampicilin
+
** stock (100 mg/mL = 100 ug/uL) ampicillin
-
** liquid culture (50ug/mL = 250ug/5mL)
+
** liquid culture (50 ug/mL = 250 ug/5mL)
-
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicilin
+
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]

Latest revision as of 09:08, 24 January 2008

Project Description / Lab Notes / Part List / Primer List


Part List

Series alias Location Description part length vector (antibotics) Check
BBa_R0082 pOmpC 1 Positively regulated, OmpR-controlled promoter 108 pSB1A2 ?
BBa_C0082 tar-envZ 1 Receptor, tar-envZ 1491 pSB2K3
BBa_B0034 RBS 1,2,3 The Elowitz RBS (Ribosome binding site) is the definition of efficiency 1.0 12 pSB1A2 ?
AU43-C7 INS human insulin 495 C
INS_A 1 alpha chain of human insulin 63
INS_B 1 beta chain of human insulin 90
BBa_B0014 D-term 1,2,3 Double transcription terminator 95 pSB1AK3 OK
BBa_I14032 pLacIQ 2 Constitutive Promoter, High Transcription 37 pSB2K3
OmpRBS 2 OmpR binding sites 65 ?
AV88-C6 IDE 2 insulin degradation enzyme of mouse 3445 A
AU61-F6 IGFBP3 2 Insulin-like growth factor binding protein 3 of human 2488 C
AU50-C7 IGFBP7 2 Insulin-like growth factor binding protein 7 of human 1155 C
BBa_I15016 B0032.ECFP 16D, plate 1 This part accepts POPS to produce ECFP under the medium RBS B0032 742 pSB1A2 OK
BBa_I15017 B0032.EYFP 16F, plate 1 This part accepts POPS to generate EYFP under the medium strength RBS B0032 742 pSB1A2 OK
BBa_E0840 B0030(RBS).E0040(GFP).B0015(D-term) 16E, plate 1 gfp tri-part; strong rbs 878 pSB1A2 OK
BBa_R0077 pCinRHSL, RBS+ 17L, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS. 231 pSB1A2 OK
BBa_R0078 pCinRHSL 15D, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS. 225 pSB1A2 ?
BBa_C0077 CinR 7G, Plate 2 cinR activator 762 pSB2K3 OK
BBa_C0076 HSL 7E, Plate 2 autoinducer 702 pSB2K3 OK

antibiotics keys