NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
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|M_INS_A 3'
|M_INS_A 3'
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|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
-
|55 bp
+
|48 bp
|54.74
|54.74
|SpeI, PstI
|SpeI, PstI
|126 bp
|126 bp
-
|Potential dimer formation!
+
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
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|M_INS_B 5'
|M_INS_B 5'
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|M_INS_B 3'
|M_INS_B 3'
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|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
-
|55 bp
+
|48 bp
|55.86
|55.86
|SpeI, PstI
|SpeI, PstI
|156 bp
|156 bp
-
|Potential dimer formation!
+
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
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|H_INS_A 3'
|H_INS_A 3'
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|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
-
|56 bp
+
|49 bp
|57.77
|57.77
|SpeI, PstI
|SpeI, PstI
|129 bp
|129 bp
-
|Potential dimer formation!
+
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
|-
|-
|H_INS_B 5'
|H_INS_B 5'
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|H_INS_B 3'
|H_INS_B 3'
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|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
-
|55 bp
+
|48 bp
|55.10
|55.10
|SpeI, PstI
|SpeI, PstI
|156 bp
|156 bp
-
|
+
|Stop codons are removed to enable fusion with export signal.
|-
|-
|-
|-

Revision as of 07:05, 23 August 2007

Name Sequence Length Tm (C) Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG 51 bp 56.85 EcoRI, XbaI 126 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 126 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 156 bp Potential dimer formation!
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 156 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG 51 bp 62.45 EcoRI, XbaI 129 bp Potential dimer formation!
H_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 129 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG 51 bp 57.98 EcoRI, XbaI 156 bp Potential dimer formation!
H_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 156 bp Stop codons are removed to enable fusion with export signal.
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1404 bp
RcsC 3'-2 GGGGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1404 bp Three extra guanosine was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
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