NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
m
Line 11: Line 11:
!Length
!Length
!Tm (C)
!Tm (C)
-
!Restriction site
+
!RE site
!Target length
!Target length
!Description
!Description
|-
|-
|M_INS_A 5'
|M_INS_A 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
+
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
-
|51 bp
+
|54 bp
|56.85
|56.85
-
|EcoRI, XbaI
+
|XbaI
|119 bp
|119 bp
-
|Potential dimer formation!
+
|Potential dimer formation! XbaI+RBS
|-
|-
|-
|-
Line 33: Line 33:
|-
|-
|M_INS_B 5'
|M_INS_B 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
+
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|52 bp
|52 bp
|60.85
|60.85
Line 51: Line 51:
|-
|-
|H_INS_A 5'
|H_INS_A 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
+
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|51 bp
|51 bp
|62.45
|62.45
|EcoRI, XbaI
|EcoRI, XbaI
|122 bp
|122 bp
-
|Potential dimer formation!
+
|Potential dimer formation! XbaI+RBS
|-
|-
|-
|-
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|-
|-
|H_INS_B 5'
|H_INS_B 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
+
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
|51 bp
|51 bp
|57.98
|57.98
|EcoRI, XbaI
|EcoRI, XbaI
|149 bp
|149 bp
-
|Potential dimer formation!
+
|Potential dimer formation! XbaI+RBS
|-
|-
|-
|-
Line 95: Line 95:
|-
|-
|RcsC 3'-2
|RcsC 3'-2
-
|<font color="green">GGGGTATAC</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
+
|<font color="green">GTTTCTTCGTATAC</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
-
|29 bp
+
|34 bp
|59.63
|59.63
|NdeI
|NdeI
|1404 bp
|1404 bp
-
|Three extra guanosine was added to the 5' side of the NdeI cutting site.
+
|BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
|-
|-
|-
|-

Revision as of 15:10, 24 August 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A 5' GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
M_INS_B 5' GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 149 bp Potential dimer formation!
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_A 5' GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTGGAACAATGCTG 51 bp 62.45 EcoRI, XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation! Stop codons are removed to enable fusion with export signal.
H_INS_B 5' GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTGAACCAACACCTGTG 51 bp 57.98 EcoRI, XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp Stop codons are removed to enable fusion with export signal.
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1404 bp
RcsC 3'-2 GTTTCTTCGTATACCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
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