NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
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|M_INS_B 5' | |M_INS_B 5' | ||
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font> | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font> | ||
| - | | | + | |55 bp |
|60.85 | |60.85 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| Line 52: | Line 52: | ||
|H_INS_A 5' | |H_INS_A 5' | ||
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font> | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font> | ||
| - | | | + | |54 bp |
|62.45 | |62.45 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
| Line 69: | Line 69: | ||
|H_INS_B 5' | |H_INS_B 5' | ||
|<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font> | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font> | ||
| - | | | + | |54 bp |
|57.98 | |57.98 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
Revision as of 15:12, 24 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTCAAGCAGCACCTTTGT | 55 bp | 60.85 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTGGAACAATGCTG | 54 bp | 62.45 | EcoRI, XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTGAACCAACACCTGTG | 54 bp | 57.98 | EcoRI, XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GTTTCTTCGTATACCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| VF2 | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| VR | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
