NYMU Taipei/Protocols2
From 2007.igem.org
(Difference between revisions)
| Line 90: | Line 90: | ||
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/29_putative_signal_peptides 29 putative signal peptides] </li> | <li>[https://2007.igem.org/NYMU_Taipei/Protocols2/29_putative_signal_peptides 29 putative signal peptides] </li> | ||
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Selected_TAT_signal_peptides Selected TAT signal peptides]</li> | <li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Selected_TAT_signal_peptides Selected TAT signal peptides]</li> | ||
| - | <li>[https://2007.igem.org/NYMU_Taipei/Protocols2/ | + | <li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Signal_peptides_and_insulin_A,_B_chain Signal peptides and insulin A, B chain]</li> |
<li>[[Signal peptides and IDE coding sequence]]</li> | <li>[[Signal peptides and IDE coding sequence]]</li> | ||
</ul> | </ul> | ||
Revision as of 05:31, 21 October 2007
Wet-Lab Protocols
- Protocol online
- PCR (7/20)
- RT-PCR
- Q-PCR
- Quantitation of Nucleic acid concentration
- [Wikipedia]
- [Pierce]
- [India]
- [Kreatech Biotechnology]
- <span lang="EN-US" style="font-size: 9.5pt; font-family: Arial [Setting Up a Restriction Endonuclease Reaction]</span>
- DNA Analysis through Gel Electrophoresis
- Ligate blunt or cohesive ends in 5 minutes at room temperature
- [Quick Ligation Kit (NEB)]
- [Fast Ligation Kit (UBI)]
- [Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]
- [Rapid Ligation Kit (Fermentas)]
- All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.
Use of crowding agents like PEG allow 5-minute sticky end ligations.
- Vector construction
- Transformation
- Production of heterologous proteins in Escherichia coli
- [Antibiotics]
- [Part fabrication] ([Enzyme selection for BioBricks digest]!!)
- [BioBricks construction tutorial]
- [Fusion protein]
- Glucose assay
- Mammalian Gene Collection (MGC) Full-length cDNA clones
Dry-Lab Protocols
- [serial cloner]
- Protein Fusion Design
- rcsC-envZ cimaera design
- Twin-Arginine Translocation Pathway Signal Peptide
- Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395
