NYMU Taipei/Protocols2
From 2007.igem.org
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| - | <li> | + | <li>[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp Setting Up a Restriction Endonuclease Reaction]</li> |
<li><font size="2">DNA Analysis through Gel Electrophoresis</font></li> | <li><font size="2">DNA Analysis through Gel Electrophoresis</font></li> | ||
<li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font> | <li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font> | ||
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<li><font size="2">[Transformation] </font></li> | <li><font size="2">[Transformation] </font></li> | ||
<li><font size="2">[https://2007.igem.org/NYMU_Taipei/Protocols2/Production_of_heterologous_proteins_in_Escherichia_coli Production of heterologous proteins in Escherichia coli] </font></li> | <li><font size="2">[https://2007.igem.org/NYMU_Taipei/Protocols2/Production_of_heterologous_proteins_in_Escherichia_coli Production of heterologous proteins in Escherichia coli] </font></li> | ||
| - | <li> | + | <li>[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks <font size="2">Antibiotics</font>]<font size="2"> </font></li> |
<li>[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication <font face="Arial" size="2">Part fabrication</font>]<font size="2"> (</font>[http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest <font size="2">Enzyme selection for BioBricks digest</font>]<font size="2">!!) </font></li> | <li>[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication <font face="Arial" size="2">Part fabrication</font>]<font size="2"> (</font>[http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest <font size="2">Enzyme selection for BioBricks digest</font>]<font size="2">!!) </font></li> | ||
<li>[http://openwetware.org/wiki/BioBricks_construction_tutorial <font size="2">BioBricks construction tutorial</font>]<font size="2"> </font></li> | <li>[http://openwetware.org/wiki/BioBricks_construction_tutorial <font size="2">BioBricks construction tutorial</font>]<font size="2"> </font></li> | ||
Latest revision as of 05:02, 27 October 2007
Wet-Lab Protocols
- Protocol online
- PCR (7/20)
- RT-PCR
- Q-PCR
- Quantitation of Nucleic acid concentration
- Setting Up a Restriction Endonuclease Reaction
- DNA Analysis through Gel Electrophoresis
- Ligate blunt or cohesive ends in 5 minutes at room temperature
- Quick Ligation Kit (NEB)
- Fast Ligation Kit (UBI)
- Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)
- Rapid Ligation Kit (Fermentas)
- All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.
Use of crowding agents like PEG allow 5-minute sticky end ligations.
- Vector construction
- [Transformation]
- Production of heterologous proteins in Escherichia coli
- Antibiotics
- Part fabrication (Enzyme selection for BioBricks digest!!)
- BioBricks construction tutorial
- Fusion protein
- Glucose assay
- Mammalian Gene Collection (MGC) Full-length cDNA clones
Dry-Lab Protocols
- serial cloner
- Protein Fusion Design
- rcsC-envZ cimaera design
- Twin-Arginine Translocation Pathway Signal Peptide
- Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395
