NYMU Taipei/Protocols2
From 2007.igem.org
Wet-Lab Protocols
- Protocol online
- PCR (7/20)
- RT-PCR
- Q-PCR
- Quantitation of Nucleic acid concentration
- Setting Up a Restriction Endonuclease Reaction
- DNA Analysis through Gel Electrophoresis
- Ligate blunt or cohesive ends in 5 minutes at room temperature
- Quick Ligation Kit (NEB)
- Fast Ligation Kit (UBI)
- Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)
- Rapid Ligation Kit (Fermentas)
- All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.
Use of crowding agents like PEG allow 5-minute sticky end ligations.
- Vector construction
- [Transformation]
- Production of heterologous proteins in Escherichia coli
- Antibiotics
- Part fabrication (Enzyme selection for BioBricks digest!!)
- BioBricks construction tutorial
- Fusion protein
- Glucose assay
- Mammalian Gene Collection (MGC) Full-length cDNA clones
Dry-Lab Protocols
- serial cloner
- Protein Fusion Design
- rcsC-envZ cimaera design
- Twin-Arginine Translocation Pathway Signal Peptide
- Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395
