NYMU Taipei/Team schedule

From 2007.igem.org

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             <td align="center"><strong>Run</strong></td>
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             <td align="center">威</td>
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<p>&nbsp;</p>
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[http://2007.igem.org/NYMU_Taipei/Welcome Back]
[http://2007.igem.org/NYMU_Taipei/Welcome Back]

Revision as of 16:32, 19 October 2007

July 2007

Sun. Mon. Tue. Wed. Thr. Fri. Sat.
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31        

 

Aug 2007

Sun. Mon. Tue. Wed. Thr. Fri. Sat.
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31  

 

System 1 assembly

 

Run Date and Time
Objective Attendance
1 8 AM Sat, 9/1, 07
  • (RBS + cinR) + (RBS + HSL) = cinR + HSL
  • RBS + cinR ver. 2
  • plate culture for (cinR + HSL) and (RBS + CinR) ver.2
茹, 翔
2 8 AM Sun, 9/2, 07 雄, 威, 茹, 駿
3 8 AM Mon, 9/3, 07
  • plasmid extraction for (cinR + HSL) and (RBS + CinR) ver.2
  • pOmpC + (RBS + INS_A)
  • (RBS + cinR) + (RBS + HSL) + D-term
宥, 君, 威
4 8 AM Tue, 9/4, 07
  • plate culture for ...
  • Measure the concentration of all excised parts
    • (cinR + HSL)
    • (RBS + CinR) ver.2
  • BsaBI digestion of cinR
宥, 君, 威
5 8 AM Wed, 9/5, 07
  • (RBS + INS_A), PCR #1
  • (RBS + INS_B), PCR #1
  • pOmpC + (RBS + INS_A), not check yet
  • pOmpC + (RBS + INS_B), not check yet
威, 茹
6 8 AM Thr, 9/6, 07
  • N/A
7 8 AM Fri, 9/7, 07
  • N/A
儒, 威, 茹
8 8 AM Sat, 9/8, 07
  • (RBS+INS_A), PCR #2
  • (RBS+INS_B), PCR #2
  • GEL check (5 tubes for each gene)
    • INS_A has two tubes A1 (0.08g), A3 (0.15g)
    • INS_B has five tubes B1 (0.12g), B2 (0.12g), B3 (0.16g), B4 (0.11g), B5 (0.17g)
  • ligation for INS_A and pOmpC
  • plate culture for (pOmpC+INS_A and pOmpC+INS_B), transformation failed
    • forget to add vector
倫, 茹
9 8 AM Sun, 9/9, 07
  • plate culture failed (possible due to absent of vector, checked by text paper record)
  • re-ligate INS_A insert into vector with pOmpC
  • re-check the concentration of insert INS_A by <a href="http://rsb.info.nih.gov/ij/">imageJ</a>
  • plate culture again for pOmpC+INS_A
    • 1uL insert + 1uL vector
倫, 翔
10 4 PM Mon, 9/10, 07
  • plate culture is still failed
  • re-build pOmpC + INS_A in two different insert concentration
    • 2uL insert + 1uL vector
    • 3uL insert + 1uL vector
倫, 翔, 銘
11 6 PM Thu, 9/11, 07
  • re-measure the concentration of insert by biophotometer
    • dsDNA use A260 OD to estimate, 1 A260 OD = 50 mg/mL = 0.05 ug/uL
    • OD ranged from 0.1 - 2.0 is located in linear region
    • OD close to zero is not reliable
  • test for D7 and D8 (9/8 PCR #2) in box #1
12 6 PM Wed, 9/12, 07
  • check (9/5 PCR) by GEL separation
    • concentration of INS_A is still too low
  • RE-examine the condition of PCR in different annealing temperature
    • PCR INS_A in 49.8, 52.3, 55.5, 58.7 and 60.9 degree
13 6 PM Thur, 9/13, 07
  • check (9/12 PCR) by GEL separation
    • band seems O.K.
    • best annealing temperature is around 55.5
  • biophotometer concentration test
    • 9/5 and 9/12 PCR seem O.K.
    • 9/8 PCR seem problemistic due to A260/A280 more than 10
14 6 PM Sun, 9/16, 07
  • digest CinR+HSL (ES) and D-term (EX)and GEL separation check
    • tube #1 and #2 of CinR+HSL plasmid extraction is not correct after GEL check
    • first digestion of D-term (EcoRI) is not very sucessful
      • thus pett re-digest D-term with double enzyme amount
宥, 君, 翔
PCR Thur, 9/27, 07
  • PCR of TAT_INS_A, TAT_INS_B, OmpRBS, TAT_IDE

 

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