NYMU Taipei/phptest
From 2007.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
<form name=form1> | <form name=form1> | ||
| - | <INPUT type="text" name="insert_size" > (bp) size of insert part<BR> | + | <INPUT type="text" name="insert_size" > (bp) size of insert part (SI)<BR> |
| - | <INPUT type="text" name="vector_size" > (bp) size of vector part<BR> | + | <INPUT type="text" name="vector_size" > (bp) size of vector part (SV)<BR> |
| - | <INPUT type="text" name="I" > (ug/uL) concentration of insert<BR> | + | <INPUT type="text" name="I" > (ug/uL) concentration of insert (CI)<BR> |
| - | <INPUT type="text" name="V" > (ug/uL) concentration of vector<BR> | + | <INPUT type="text" name="V" > (ug/uL) concentration of vector (CV)<BR> |
| - | <INPUT type="text" name="X" > volume (uL) of insert for gel separation<BR> | + | <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR> |
| - | <INPUT type="text" name="L" > volume (uL) of insert for ligation<BR> | + | <INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> |
<input type=button value="送出" Onclick="CheckId(insert_size.value, vector_size.value);"> | <input type=button value="送出" Onclick="CheckId(insert_size.value, vector_size.value);"> | ||
</form> | </form> | ||
| - | |||
| - | |||
| - | |||
| - | |||
</body> | </body> | ||
</html> | </html> | ||
| + | ---- | ||
| + | * vector weight is 300 ng | ||
| + | * total digestion volume is 20 uL (typical) | ||
| + | ** enzyme 1 and enzyme 2 (1uL * 2) | ||
| + | ** 10X buffer (2uL) | ||
| + | ** BSA (2uL) | ||
| + | * total gel separation volume is 50 uL | ||
| + | ---- | ||
| + | * formula | ||
| + | ** (1) 300 * SI * 3 * 10 / SV = CI * X * L /50 | ||
| + | ** (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV | ||
| + | ** VV is volume of vector | ||
Revision as of 06:56, 16 September 2007
- vector weight is 300 ng
- total digestion volume is 20 uL (typical)
- enzyme 1 and enzyme 2 (1uL * 2)
- 10X buffer (2uL)
- BSA (2uL)
- total gel separation volume is 50 uL
- formula
- (1) 300 * SI * 3 * 10 / SV = CI * X * L /50
- (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
- VV is volume of vector
