NYMU Taipei/phptest

From 2007.igem.org

(Difference between revisions)
Line 4: Line 4:
<script language="javascript">
<script language="javascript">
-
function CheckId(v1,v2){
+
function CheckId(SI,SV){
-
var a = v1;
+
var SI = SI;
-
var b = v2;
+
var SV = SV;
//document.write("insert is v1, and vector is v2");
//document.write("insert is v1, and vector is v2");
-
alert("insert size is " + a + " \nand vector size is " + b);
+
alert("insert size is " + SI + " \nand vector size is " + SV);
}
}
Line 17: Line 17:
<form name=form1>
<form name=form1>
-
     <INPUT type="text" name="insert_size" >  size (bp) of insert part (SI)<BR>
+
     <INPUT type="text" name="SI" >  size (bp) of insert part (SI)<BR>
-
     <INPUT type="text" name="vector_size" >  size (bp) of vector part (SV)<BR>
+
     <INPUT type="text" name="SV" >  size (bp) of vector part (SV)<BR>
     <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR>
     <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR>
     <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR>
     <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR>
     <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
     <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
-
     <input type=button value="送出" Onclick="CheckId(insert_size.value, vector_size.value);">
+
     <input type=button value="送出" Onclick="CheckId(SI.value, SV.value);">
</form>
</form>
</body>
</body>

Revision as of 07:01, 16 September 2007

size (bp) of insert part (SI)
size (bp) of vector part (SV)
concentration (ug/uL) of insert (CI)
concentration (ug/uL) of vector (CV)
volume (uL) of insert for gel separation (VI)
volume (uL) of insert for ligation (L)


  • total digestion volume is 20 uL (typical)
    • enzyme 1 and enzyme 2 (1uL * 2)
    • 10X buffer (2uL)
    • BSA (2uL)
    • plasmid + ddH2O (14uL)

  • total gel separation volume is 50 uL
    • vector weight is 300 ng

  • formula
    • (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
    • (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
    • VV (uL) is volume of vector
    • CV (ng/uL) is concentration of vector