NYMU Taipei/phptest

From 2007.igem.org

(Difference between revisions)
Line 19: Line 19:
     <INPUT type="text" name="SI" >  size (bp) of insert part (SI)<BR>
     <INPUT type="text" name="SI" >  size (bp) of insert part (SI)<BR>
     <INPUT type="text" name="SV" >  size (bp) of vector part (SV)<BR>
     <INPUT type="text" name="SV" >  size (bp) of vector part (SV)<BR>
-
     <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR>
+
     <INPUT type="text" name="CI" > concentration (ug/uL) of insert (CI)<BR>
-
     <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR>
+
     <INPUT type="text" name="CV" > concentration (ug/uL) of vector (CV)<BR>
-
     <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
+
     <INPUT type="text" name="VI" > volume (uL) of insert for gel separation (VI)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
     <input type=button value="送出" Onclick="CheckId(SI.value, SV.value);">
     <input type=button value="送出" Onclick="CheckId(SI.value, SV.value);">
Line 32: Line 32:
** 10X buffer (2uL)
** 10X buffer (2uL)
** BSA (2uL)
** BSA (2uL)
-
** plasmid + ddH2O (14uL)
+
** plasmid + ddH2O (14uL = VI + water, 1 <= VI <= 14)
----
----
* total gel separation volume is 50 uL
* total gel separation volume is 50 uL

Revision as of 07:03, 16 September 2007

size (bp) of insert part (SI)
size (bp) of vector part (SV)
concentration (ug/uL) of insert (CI)
concentration (ug/uL) of vector (CV)
volume (uL) of insert for gel separation (VI)
volume (uL) of insert for ligation (L)


  • total digestion volume is 20 uL (typical)
    • enzyme 1 and enzyme 2 (1uL * 2)
    • 10X buffer (2uL)
    • BSA (2uL)
    • plasmid + ddH2O (14uL = VI + water, 1 <= VI <= 14)

  • total gel separation volume is 50 uL
    • vector weight is 300 ng

  • formula
    • (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
    • (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
    • VV (uL) is volume of vector
    • CV (ng/uL) is concentration of vector