Paris/August 1

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DGAT Assembly PCR: Megaprimer PCR

Material:

- DNA plasmid (pKS::DGAT): 1.5µL (200ng) - Tampon: 10µL - dNTP: 1µL (final 200µmol each) - Taq: 0.5µL - Water: 29µL - Primer: ForDGAT1 (): 4µL - Megaprimer: DGAT2: 4µL

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